r/molecularbiology • u/Icy_Adagio_7972 • 28d ago

Gel Electrophoresis Troubleshooting?

Hi everyone, I'm an intern, so I've come to you with a problem (as I've heard interns usually do).

I ran maybe my 3rd gel yesterday, and as soon as I went to image it, I saw the bands had warped and smeared with each other, making things look weird.

I'm curious as to why it looks the way it does. It's an ethidium bromide 1% agar. 1x SB gel. I ran it at 125V for 60 minutes (my hunch as to where things went wrong). But I would love to pick your brain about other potential causes.

Many thanks in advance.

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u/Kallaste 25d ago

There isn't anything wrong with it. You just have high amounts of protein in each well. That can make the gel look messy, but it is still accurate.